The Islamic Revolutionary Guard Corps said the jet was hit at 2:50 AM on 19 March in central Iran using advanced air defense systems.
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It said the jet’s fate is unclear and under investigation, and the likelihood of a crash is very high.
US officials said the F-35 made an emergency landing after a missile barrage, with the pilot safe and an investigation underway.
The claim came after the White House said the F-35 represents “decisive American power” and, if confirmed, would mark the first such hit in the war.